孙前文 博士

副教授

2022-今 副教授，beat365官方网站

2014-2021   助理教授， beat365官方网站

2014-今 研究员，清华-北大生命科学联合中心

2009-2014    博士后，John Innes Centre, Norwich, UK

2004-2009    博士，华中农业大学 生科院

2000-2004    学士，华中农业大学 生科院

●  研究兴趣、领域：

我们实验室感兴趣各个层面的基因组调控事件，重点关注R-loop生物学研究与基因组调控。R-loop是由一条单链DNA和另一条RNA:DNA杂合链混合组成的三股染色质结构，在许多生物体中发挥着重要的生物功能。我们以拟南芥和其它模式生物（如大肠杆菌和酵母）为模型来研究R-loop生物学的基本问题，如R-loop形成、稳定与解除的分子机理，以及R-loop如何影响转录调控和基因组维持。

 

●  已发表论文：

(* corresponding or co-corresponding authors; # co-first authors)

1.     Weifeng Zhang, Zhuo Yang, Wenjie Wang and Qianwen Sun*. Primase promotes the competition between transcription and replication on the same template strand resulting in DNA damage. Nature Communications. 2024; 15, 73. https://doi.org/10.1038/s41467-023-44443-0.

2.     Qin Li, Jincong Zhou, Shuai Li, Weifeng Zhang, Yingxue Du, Kuan Li, Yingxiang Wang and Qianwen Sun*. DNA polymerase ε harmonizes topological states and R-loops formation to maintain genome integrity in Arabidopsis. Nature Communications. 2023; 14, 7763. https://doi.org/10.1038/s41467-023-43680-7.

3.     Jincong Zhou, Xue Lei, Sarfraz Shafiq, Weifeng Zhang, Qin Li, Kuan Li, Jiafu Zhu, Zhicheng Dong, Xin-Jian He and Qianwen Sun*. DDM1-mediated R-loop resolution and H2A.Z exclusion facilitates heterochromatin formation in Arabidopsis. Science Advances. 2023 Aug;9(32):eadg2699. doi: 10.1126/sciadv.adg2699.

4.     Wei Xu#*, Chao Liu#, Zhe Zhang#, Changbin Sun, Qin Li, Kuan Li, Hui Jiang*, Wei Li* and Qianwen Sun*. DEtail-seq is an ultra-efficient and convenient method for meiotic DNA break profiling in multiple organisms. Sci China Life Sci, 2023 June; 66(6): 1392–1407. https://doi.org/10.1007/s11427-022-2277-y.

5.     Kuan Li#, Zhenzhen Wu#, Jincong Zhou#, Wei Xu, Ling Li, Chao Liu, Wei Li, Chengjun Zhang* and Qianwen Sun*. R-loopAtlas: An integrated R-loop resource from 254 plant species sustained by a deep-learning-based tool. Molecular Plant. 2023 Mar;16(3):493-496. doi: 10.1016/j.molp.2022.12.012.

6.       Liu K and Sun Q*. Intragenic tRNA-promoted R-loops orchestrate transcription interference for plant oxidative stress responses. The Plant Cell, 2021, 33: 3574–3591.

7.       Cheng L#, Wang W#, Yao Y#, Sun Q*. Mitochondrial RNase H1 activity regulates R-loop homeostasis to maintain genome integrity and enable early embryogenesis in Arabidopsis. PLOS Biology, 2021, 19(8): e3001357.

8.       Wang W, Li K, Yang Z, Hou Q, Zhao W W, Sun Q*. RNase H1C collaborates with ssDNA binding proteins WHY1/3 and recombinase RecA1 to fulfill the DNA damage repair in Arabidopsis chloroplasts. Nucleic Acids Research, 2021 Jul; 49(12): 6771–6787.

9.       Yang Z, Li M, Sun Q*. RHON1 Co-transcriptionally Resolves R-Loops for Arabidopsis Chloroplast Genome Maintenance. Cell Reports. 2020 Jan; 30(1), 243–256.

10.       Xu W#, Li K#, Li S#, Hou Q#, Zhang Y, Liu K and Sun Q*. The R-loop atlas of Arabidopsis development and responses to environmental stimuli. The Plant Cell, 2020 Apr; 32(4):888-903.

11. Zhao S#, Cheng L#, Gao Y#, Zhang B#, Zheng X, Wang L, Li P*, Sun Q*, Li H*. Plant HP1 protein ADCP1 links multivalent H3K9 methylation readout to heterochromatin formation. Cell Research, 2019 Jan; 29(1):54-66.

12.       Yang Z#, Hou Q#, Cheng L#, Xu W, Hong Y, Li S, Sun Q*. RNase H1 Cooperates with DNA Gyrases to Restrict R-Loops and Maintain Genome Integrity in Arabidopsis Chloroplasts. The Plant Cell, 2017 Oct; 29(10):2478-2497.

13.       Xu W, Xu H, Li K, Fan Y, Liu Y, Yang X*, Sun Q*. The R-loop is a common chromatin feature of the Arabidopsis genome. Nature Plants, 2017 Sep; 3(9):704-714.

14.       Sun Q, Csorba T, Skourti-Stathaki K, Proudfoot NJ, Dean C*. R-loop stabilization represses antisense transcription at the Arabidopsis FLC locus. Science, 2013 May; 340(6132):619-21.

●  联系方式：

地址：清华大学生物技术馆4段313室(办公室)，309室(实验室)

电话:  +86-10-62784002 (办公室); 62796360 (实验室)

电子邮件：sunqianwen(a t)mail.tsinghua.edu.cn