Xueming LI Ph.D.
Associate Professor
1998-2002 B.S. University of Science and Technology Beijing
2002-2005 M.S. University of Science and Technology Beijing
2005-2009 Ph.D. Institute of Physics, CAS
2009-2013 Postdoc University of California, San Francisco
2014-present Assistant professor, Associate professor, School of Life Sciences, Tsinghua University
2014-present PI, Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University
Beijing Frontier Research Center for Biological Structure, Tsinghua University
Research interest
We engaged in the theoretical and methodological research of electron cryo-microscopy (CryoEM) and its application in structural biology to address important biological questions. We are focusing on developing and applying novel image processing and experimental technologies to advance CryoEM for routine atomic-resolution structure analysis of biological structures. In the future, we hope to develop advanced experimental and image processing and analysis technologies, as well as artificial intelligence technologies, to reveal nanometer and even atomic resolution structures of tissue and cell, so that we can understand the essence of life activities at the atomic or molecular level, and promote the application of cryo-electron microscopy technology in fields such as drug design and medical care.
Selected publications
1. Chao Lin#, Li Zhang#, Ziying Zhang#, Yifeng Jiang#, Xueming Li*. Locating cellular contents during cryoFIB milling using cellular secondary-electron imaging. Journal of Structural Biology, 2023, 215, 108005.
2. Sihan Wang#, Heng Zhou#, Wei Chen#, Yifeng Jiang, Xuzhen Yan, Hong You*, Xueming Li*. CryoFIB milling large tissue samples for cryo-electron tomography. Scientific Reports, 2023,13, 5879.
3. Xinyu Zhang#, Tianfang Zhao#, Jiansheng Chen*, Yuan Shen*, Xueming Li*, EPicker is an exemplar-based continual learning approach for knowledge accumulation in cryoEM particle picking, Nature Communications, 2022, 13, 2468
4. Xuzhong Yang#, Chao Lin#, Xudong Chen#, Shouqin Li#, Xueming Li*, Bailong Xiao*, Structure deformation and curvature sensing of PIEZO1 in lipid membranes, Nature, 2022, 604, 377-383
5. Mingxu Hu#, Qi Zhang#, Jing Yang*, Xueming Li*, Unit quaternion description of spatial rotations in 3D electron cryo-microscopy, Journal of Structural Biology, 2020, 212, 107601
6. Zhaofeng Yan#, Meng Yin#, Jianan Chen#, Xueming Li*, Assembly and Substrate Recognition of Curli Biogenesis System, Nature Communication, 2020, 11，241
7. Heng zhou#, Feng Luo#, Zhipu Luo#, Dan Li, Cong Liu*, Xueming Li*, Programming conventional electron microscopes for solving ultrahigh-resolution structures of small and macro-molecules, Analytical Chemistry, 2019, 91, 10996-11003
8. Meijing Li#, Xian Xia#, Yuanyuan Tian#, Qi Jia#, Xiaoyu Liu#, Ying Lu, Ming Li*, Xueming Li*, and Zhucheng Chen*, Mechanism of DNA translocation underlying chromatin remodeling by Snf2, Nature, 2019, 567, 409-413
9. Heng Zhou#, Zhipu Luo#, Xueming Li*, Using Focus Ion Beam to Prepare Crystal Lamella for Electron Diffraction, Journal of Structural Biology, 2019, 205, 59-64
10. Mingxu Hu#, Hongkun Yu#, Kai Gu#, Zhao Wang, Huabin Ruan, Kunpeng Wang, Siyuan Ren, Bing Li, Lin Gan, Shizhen Xu, Guangwen Yang*, Yuan Shen*, Xueming Li*, A particle-filter framework for robust cryoEM 3D reconstruction, Nature Methods, 2018, 15, 1083-1089
Contact information
http://thuem.net
