Jiawei WANG     Ph.D.

Associate  Professor

2001  University of Science and Technology of China／B.S.

2005  Institute of Physics, Chinese Academy of Sciences ／Ph .D.

2005－2008 Argonne National Laboratory, USA／Postdoctoral Fellow

2008－2011 School of Life Sciences, Tsinghua University／Senior Engineer

2011－2017 School of Life Sciences, Tsinghua University／Assistant Professor

2017-present School of Life Sciences, Tsinghua University, China／Associate Professor

 

Research interest

Mannose transport complex (man-PTS) is an important member of bacterial phosphotransferase system (PTS), which is responsible for the uptake of glucose, mannose and various sugars. In addition, man-PTS has been reported as a target of related bacteriocins, which participate in or mediate the bactericidal effect. Our team has analyzed the structure of man-PTS from Escherichia coli, the target of bacteriocin. In the future, we will continue to study the bactericidal mechanism of bacteriocins and man-PTS by means of crystallography and cryo- electron microscopy, and provide ideas for the design and development of new antibacterial drugs and food additives. At the same time, we explore the difficult problems in the process of structural analysis and develop new structural analysis methods.

Selected publications

1. Li R, Duan J, Zhou Y, Wang J*. Structural basis of the mechanisms of action and immunity of lactococcin A, a class IId bacteriocin. Appl Environ Microbiol. 2023; 89:e00066-23.

2. Zhu L, Zeng J, Wang J*. Structural basis of the immunity mechanisms of pediocin-like bacteriocins. Appl Environ Microbiol. 2022; 88(13):e0048122.

3. Wang C, Zeng J*, Wang J*. Structure basis of bacteriophage lambda capsid maturation. Structure, 2022; S0969-2126(21)00461-5.

4. Zhu L, Zeng J, Wang C, Wang J*. Structural basis of pore formation in the mannose phosphotransferase system by pediocin PA-1. Appl Environ Microbiol. 2022; 88(3):e0199221.

5. Huang K, Zeng J, Liu X, Jiang T*, Wang J*. Structure of the mannose phosphotransferase system (man-PTS) complexed with microcin E492, a pore-forming bacteriocin. Cell Discovery, 2021;7(1):20.

6. Li L, Dai S, Gao GF*, Wang J*. Lattice-translocation defects in specific crystals of the catalytic head domain of influenza neuraminidase. Acta Crystallogr D Struct Biol. 2020;76:1057-1064 (Cover).

7. Su R*, Zhang S, Wang J*. Automatically generating mask for mapping out the density map in cryoEM single-particle reconstruction. Progress in Biochemistry and Biophysics, 2019; 46:1020-1030.

8. Liu X, Zeng J, Huang K, Wang J*. Structure of the mannose transporter of the bacterial phosphotransferase system. Cell Research 2019;29(8):680-682.

9. Qi X, Schmiege P, Coutavas E, Wang J*, Li X*. Structures of human Patched and its complex with native palmitoylated sonic hedgehog. Nature. 2018;7716:128-132.

10. Liu X, Wang J*, Sun L*. Structure of the hypersomolality-gated calcium-permeable channel OSCA1.2. Nat Commun. 2018; 9(1):5060.

11. Zhou N, Wang H*, Wang J*. EMBuilder: A Template Matching-based Automatic Model-building Program for High-resolution Cryo-Electron Microscopy Maps. Sci Rep. 2017;7(1):2664.

12. Wang J*, Chai J, Wang H. Structure of the mouse Toll-like receptor 13 ectodomain in complex with a conserved sequence from bacterial 23S ribosomal RNA. FEBS J. 2016;283(9):1631-5 (Cover).

13. Wang J, Wang W, Song W, Han Z, Zhang H, Chai J*, Wang H*, Wang J*. An improved method for phasing crystal structures with low non-crystallographic symmetry using cryo-electron microscopy data. Protein Cell. 2015;6(12):919-23.

14. Luo P, Dai S, Zeng J, Duan J, Shi H, Wang J*. Inward-facing conformation of L-ascorbate transporter suggests an elevator mechanism. Cell Discovery, 2018;4:35.

Luo P, Yu X, Wang W, Fan S, Li X*, Wang J*. Crystal structure of a phosphorylation-coupled vitamin C transporter. Nat Struct Mol Biol. 2015;22(3):238-41 (Cover).

Contact information

Tel: +86-10-62782124

Fax: +86-10-62782124

E-mail: jwwang@tsinghua.edu.cn

Address: A317, Biomedicine Hall, Tsinghua University